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PurposeReporter assay to monitor the transcription activity of the UCP1 promoter by GFP expression
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Depositing Lab
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Depositing OrganizationWashington University in Saint Louis
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104585 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 104585-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 104585-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCMV6-AC-GFP
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Backbone manufacturerOrigene
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUCP1 promoter
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SpeciesM. musculus (mouse)
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Insert Size (bp)5500
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Entrez GeneUcp1 (a.k.a. Slc25a7, Ucp)
- Promoter UCP1
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Tag
/ Fusion Protein
- TurboGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsrGI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer unknown
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The CMV promoter driving GFP expression was removed and replaced with the -5.5 kb mouse UCP1 promoter + Kozak
Reference sequence for promoter may be found here:
http://epd.vital-it.ch/cgi-bin/get_doc?db=mmEpdNew&format=genome&entry=Ucp1_1
DNA (Catalog # 104585-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 104585-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
-5.5kb-UCP1-GFP was a gift from Irfan Lodhi (Addgene plasmid # 104585 ; http://n2t.net/addgene:104585 ; RRID:Addgene_104585) -
For your References section:
PexRAP Inhibits PRDM16-Mediated Thermogenic Gene Expression. Lodhi IJ, Dean JM, He A, Park H, Tan M, Feng C, Song H, Hsu FF, Semenkovich CF. Cell Rep. 2017 Sep 19;20(12):2766-2774. doi: 10.1016/j.celrep.2017.08.077. 10.1016/j.celrep.2017.08.077 PubMed 28930673
