pACYCduet-mxLOX2-EH
(Plasmid #104979)

• Purpose: Biosynthesis of oxylipins by microbial enzymes
• Depositing Lab: Deokkun Oh
• Publication: Bacterial expression plasmids of microbial lipoxygenases originated from Myxococcus xanthus (unpublished)

Backbone

• Vector backbone: pACYCDuet
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 4008
• Total vector size (bp): 6914
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: MXAN_1744
• Alt name: mxLOX2
• Species: Myxococcus xanthus
• Insert Size (bp): 2028
• GenBank ID: ABF 88826.1
• Entrez Gene: MXAN_1744 (a.k.a. MXAN_1744)
• Promoter: T7 promoter

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: GAATTCATGACTGTCGAGTACAAAC
• 3′ sequencing primer: TACAAGCTTTCAGACGGTGATGCCG

Gene/Insert 2

• Gene/Insert name: MXAN_1644
• Alt name: mxEH
• Species: Myxococcus xanthus
• Insert Size (bp): 957
• GenBank ID: ABF 91519.1
• Entrez Gene: MXAN_1644 (a.k.a. MXAN_1644)
• Promoter: T7 promoter

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: GCGCATATGGCTGACATCACGCATCGAACCGT
• 3′ sequencing primer: GCGCTCGAGTCAGGCCGGCAGCTTCTTCA

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequencing data found D273G in the MXAN_1744 translation, that does not affect protein function