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pFA6a-kanMX6-P3nmt1-mEGFP
(Plasmid #105137)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 105137 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6a
  • Backbone size w/o insert (bp) 4170
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    KanMX6 (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    kanMX6-P3nmt1-mEGFP
  • Alt name
    monomeric enhanced GFP
  • Alt name
    3nmt1
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    1900
  • Mutation
    A206K
  • Promoter 3nmt1
  • Tag / Fusion Protein
    • 3nmt1-mEGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site AscI (not destroyed)

Resource Information

Depositor Comments

it is more thermal stable than GFP(S65T) The sizes of backbone and inserts are approximate, and do not include the selectable marker.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-kanMX6-P3nmt1-mEGFP was a gift from Jian-Qiu Wu (Addgene plasmid # 105137 ; http://n2t.net/addgene:105137 ; RRID:Addgene_105137)
  • For your References section:

    alpha-Actinin and fimbrin cooperate with myosin II to organize actomyosin bundles during contractile-ring assembly. Laporte D, Ojkic N, Vavylonis D, Wu JQ. Mol Biol Cell. 2012 Aug;23(16):3094-110. doi: 10.1091/mbc.E12-02-0123. Epub 2012 Jun 27. 10.1091/mbc.E12-02-0123 PubMed 22740629