PurposeYeast gene targeting
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||105157||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4170
Vector typeBacterial Expression, Yeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt nametandem dimer Tomato
Insert Size (bp)1400
/ Fusion Protein
- tdTomato (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site PmeI (not destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The sizes of backbone and inserts are approximate, and do not include the selectable marker.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFA6a-tdTomato-hphMX6 was a gift from Jian-Qiu Wu (Addgene plasmid # 105157 ; http://n2t.net/addgene:105157 ; RRID:Addgene_105157)
For your References section:Cooperation between Rho-GEF Gef2 and its binding partner Nod1 in the regulation of fission yeast cytokinesis. Zhu YH, Ye Y, Wu Z, Wu JQ. Mol Biol Cell. 2013 Oct;24(20):3187-204. doi: 10.1091/mbc.E13-06-0301. Epub 2013 Aug 21. 10.1091/mbc.E13-06-0301 PubMed 23966468