b2AR->M71-IRES-tauGFP-ACNF TV
(Plasmid
#105198)
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Purposetargeting vector to generate a replacement of CDS of M71 with CDS of mouse beta2-adrenergic receptor with expression of tauGFP.
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Depositing Lab
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Depositing OrganizationMax Planck Research Unit for Neurogenetics
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105198 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 105198-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 105198-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBluescript-II-SK
- Backbone size w/o insert (bp) 3000
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Vector typeMouse Targeting
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T3
- 3′ sequencing primer T7
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Linearize the TV with PmeI for electroporation.
DNA (Catalog # 105198-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 105198-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
b2AR->M71-IRES-tauGFP-ACNF TV was a gift from Peter Mombaerts (Addgene plasmid # 105198 ; http://n2t.net/addgene:105198 ; RRID:Addgene_105198) -
For your References section:
The beta2-adrenergic receptor as a surrogate odorant receptor in mouse olfactory sensory neurons. Omura M, Grosmaitre X, Ma M, Mombaerts P. Mol Cell Neurosci. 2014 Jan;58:1-10. doi: 10.1016/j.mcn.2013.10.010. Epub 2013 Nov 6. 10.1016/j.mcn.2013.10.010 PubMed 24211702
Map uploaded by the depositor.
