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pIL2RmCherry
(Plasmid #105317)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105317 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 105317-D50 50 μg of DNA in Tris buffer $495
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DNA 105317-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pIL2RNB
  • Backbone manufacturer
    modified pEGFP-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    700

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site Xba1 (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pIL2R was constructed by replacing EGFP with transmembrane domain of IL2R using Nhe1 and BglII sites. The mCherry sequence was inserted into HindIII and Xba1 sites of pIL2R NBC1. mCherry was from Dr. Roger Tsien (UCSD).

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIL2RmCherry was a gift from Kenneth Yamada (Addgene plasmid # 105317 ; http://n2t.net/addgene:105317 ; RRID:Addgene_105317)

  • For your References section:

    Dynamic membrane remodeling at invadopodia differentiates invadopodia from podosomes. Artym VV, Matsumoto K, Mueller SC, Yamada KM. Eur J Cell Biol. 2011 Feb-Mar;90(2-3):172-80. doi: 10.1016/j.ejcb.2010.06.006. Epub 2010 Jul 24. 10.1016/j.ejcb.2010.06.006 PubMed 20656375
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