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pD765 FDFT1 3'UTR
(Plasmid #105765)

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Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105765 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 105765-D50 50 μg of DNA in Tris buffer $495
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DNA 105765-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pDEST 765
  • Backbone manufacturer
    Promega, psiCheck2
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FDFT1 3'UTR
  • Species
    H. sapiens (human)
  • Mutation
    None
  • Entrez Gene
    FDFT1 (a.k.a. DGPT, ERG9, SQS, SS)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

3'UTR clones downstream of Renilla luciferase reporter gene

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pD765 FDFT1 3'UTR was a gift from Joseph Ziegelbauer (Addgene plasmid # 105765 ; http://n2t.net/addgene:105765 ; RRID:Addgene_105765)

  • For your References section:

    Viral microRNAs Target a Gene Network, Inhibit STAT Activation, and Suppress Interferon Responses. Ramalingam D, Ziegelbauer JM. Sci Rep. 2017 Jan 19;7:40813. doi: 10.1038/srep40813. 10.1038/srep40813 PubMed 28102325
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