Purpose(Empty Backbone) High copy HRPT backbone plasmid for insertion of MiniP promoters
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||105871||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerSimpson lab
Growth in Bacteria
Copy numberHigh Copy
- 5′ sequencing primer pTF3 (5′-CTCGAAAATAATAAAGGGAAAATCAG-3′)
- 3′ sequencing primer pTR (5'-TGGTAGTGTGGGGACTC-3') (Common Sequencing Primers)
Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid, including the MiniPromoter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEMS2001 was a gift from Elizabeth Simpson (Addgene plasmid # 105871 ; http://n2t.net/addgene:105871 ; RRID:Addgene_105871)
For your References section:Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain; Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM. Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 10.1534/genetics.119.301984. 10.1534/genetics.119.301984 PubMed 30765420