Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid pAAV.CAG.SF-iGluSnFR.A184V with the following enzymes: SacII and SalI-HF.
SacII: 4 kb & 2.5 kb
SalI-HF: 5.7 kb & 0.8 kb
SacII/ SalI-HF: 3.9 kb, 1.8 kb, 0.7 kb, & 0.1 kb (not visible)
We observe the expected bands for this digest.