hASXL(1-115)-luciferase
(Plasmid #106427)

• Purpose: Expresses human ASXL1 (amino acids 1-115) fused to the N-terminal of firefly luciferase
• Depositing Lab: Douglas Feldman
• Publication: Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19)30222-9. doi: 10.1016/j.molcel.2019.03.018.

Backbone

• Vector backbone: pcDNA3.1-luciferase
• Backbone manufacturer: Addgene #18964
• Backbone size w/o insert (bp): 7041
• Total vector size (bp): 7400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: addition sex combs like 1 ASXL1
• Alt name: ASXL
• Species: H. sapiens (human)
• Insert Size (bp): 350
• Mutation: amino acids 1-115 fused to luciferase
• Entrez Gene: ASXL1 (a.k.a. BOPS, MDS)
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMF-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry