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Addgene

pBabe GFP Small T antigen
(Plasmid #10673)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10673 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBABE GFP
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SV40 small T Antigen
  • Species
    simian virus
  • Insert Size (bp)
    525
  • Entrez Gene
    SV40gp7 (a.k.a. SV40gp7)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

small T antigen expressed in pBABE vector with GFP instead of puromycin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe GFP Small T antigen was a gift from William Hahn (Addgene plasmid # 10673 ; http://n2t.net/addgene:10673 ; RRID:Addgene_10673)
  • For your References section:

    Transformation of human and murine fibroblasts without viral oncoproteins. Boehm JS, Hession MT, Bulmer SE, Hahn WC. Mol Cell Biol. 2005 Aug . 25(15):6464-74. 10.1128/MCB.25.15.6464-6474.2005 PubMed 16024784