pX552-SpCas9 sgRNA-YFP sgRNA2
(Plasmid
#107029)
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PurposepX552-U6-SpCas9 sgRNA-U6-YFP sgRNA2-hSyn1-mCherry, Kamikaze CRISPR/Cas plasmid with two guide RNAs, targeting both SpCas9 and YFP (clone 2), under control of two independent U6 promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107029 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV
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Vector typeMammalian Expression, AAV, CRISPR
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Selectable markersmCherry
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpCas9 sgRNA
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gRNA/shRNA sequenceSpCas9 sgRNA:CAGAGTTGGTGCCGATGTCC
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
one more insert: YFP sgRNA2 (CGAGGAGCTGTTCACCGGGG)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pX552-SpCas9 sgRNA-YFP sgRNA2 was a gift from Alex Hewitt (Addgene plasmid # 107029)
