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Alex Hewitt Lab: Alex Hewitt Lab plasmids
Unpublished

Plasmids from Article

ID Plasmid Purpose  
107023pX552-GFPpX552-CMV-GFP. AAV plasmid for sgRNA cloning, with GFP fusion facilitating FACS sorting of cells. Replaced the hSyn promoter in pX552 from Zhang lab with a CMV promoter.
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107024pX551-CMV-SpCas9AAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang lab
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107031pX551-miniCMV-SpCas9AAV plasmid expressing Cas9 under control of miniCMV promoter
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107032pX551-miniCMV-SaCas9AAV plasmid expressing SaCas9 under control of miniCMV promoter
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107033pX551-miniCMV-CjCas9AAV plasmid expressing CjCas9 under control of miniCMV promoter
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107034pX551-miniCMV-AsCpf1AAV plasmid expressing AsCpf1 under control of miniCMV promoter
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107035pX551-CMV-CjCas9AAV plasmid expressing CjCas9 under control of CMV promoter
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107049pX601-miniCMV-SaCas9-U6-LacZvsSaCas9 sgRNA All-in-one vectors expressing both SaCas9 and LacZ sgRNA
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107050pX601-miniCMV-SaCas9-U6-YFPvsSaCas9 sgRNA All-in-one vectors expressing both SaCas9 and YFP sgRNA
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107055 pX601 miniCMV-SaCas9-SpA-sgRNA scaffoldBackbone vector for cloning in target sgRNA for use with SaCas9
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