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pAGH50
(Plasmid #107254)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 107254 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM-3ZfP
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3170
  • Total vector size (bp) 8170
  • Modifications to backbone
    cloned the insert by Gibson into BamHI/SphI linearized vector backbone
  • Vector type
    Bacterial Expression ; suicide vector for destined for double homologous recombination of the insert

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    vipp1-gfp-fkbp12
  • Species
    Synthetic; Synechocystis sp. PCC6803
  • Insert Size (bp)
    5330
  • Promoter native
  • Tag / Fusion Protein
    • GFP and FKBP12 (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

the construct is similar to pAGH42 except that FKBP12 encoding fragment was fused in frame with Vipp1-GFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAGH50 was a gift from Erin O'Shea (Addgene plasmid # 107254 ; http://n2t.net/addgene:107254 ; RRID:Addgene_107254)
  • For your References section:

    Dynamical localization of a thylakoid membrane binding protein is required for acquisition of photosynthetic competency. Gutu A, Chang F, O'Shea EK. Mol Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912. 10.1111/mmi.13912 PubMed 29357135