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Addgene

pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-TALETS3
(Plasmid #107301)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107301 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCSDest2
  • Backbone size w/o insert (bp) 4078
  • Total vector size (bp) 9432
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Truncated SpCas9 (aa 574-1368) fused to FKBP and TALE
  • Species
    Synthetic; S. pyogenes
  • Insert Size (bp)
    5336
  • Mutation
    Truncated SpCas9 (aa 574-1368) with R1335K mutation
  • Promoter CMV IE94
  • Tags / Fusion Proteins
    • FKBP (N terminal on insert)
    • SV40NLS, 3xHA, c-Myc NLS (C terminal on insert)
    • TALE_VEGFA-TS3 (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-TALETS3 was a gift from Scot Wolfe (Addgene plasmid # 107301 ; http://n2t.net/addgene:107301 ; RRID:Addgene_107301)