VGF8a
(Plasmid #107572)

• Purpose: partial cDNA of a neurotrophin responsive gene
• Depositing Labs: Andrea Levi, Bruce Paterson
• Publication: Levi et al Science. 1985 Jul 26;229(4711):393-5.

Backbone

• Vector backbone: pUC
• Vector type: Cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VGF8a
• Alt name: NM_030997
• Species: R. norvegicus (rat)
• Mutation: partial cDNA (amino acid 65 - 617)
• Entrez Gene: Vgf

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: The VGF insert contains a V480M mutation compared to the closest reference sequence. The depositor is unable to confirm what, if any, functional impact this has on the plasmid.