pEXP(V5-APEX2-NES)
(Plasmid
#107596)
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PurposeExpresses V5-APEX2-NES in mammalian cells
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Depositing Lab
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Depositing OrganizationNational Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107596 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 107596-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 107596-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepDEST
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Modifications to backboneQuite a few
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Vector typeMammalian Expression ; FRT site to insert into HEK293 T-REx genome
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAPEX2-NES
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SpeciesSynthetic
- Promoter CMV Dox inducible
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Tags
/ Fusion Proteins
- NES (C terminal on insert)
- V5 (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMVf
- 3′ sequencing primer BGH
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypcDNA3 APEX2-NES (Addgene plasmid 49386) by Alice Ting
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 107596-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 107596-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEXP(V5-APEX2-NES) was a gift from Markus Hafner (Addgene plasmid # 107596 ; http://n2t.net/addgene:107596 ; RRID:Addgene_107596) -
For your References section:
Proximity-CLIP provides a snapshot of protein-occupied RNA elements in subcellular compartments. Benhalevy D, Anastasakis DG, Hafner M. Nat Methods. 2018 Dec;15(12):1074-1082. doi: 10.1038/s41592-018-0220-y. Epub 2018 Nov 26. 10.1038/s41592-018-0220-y PubMed 30478324
Map uploaded by the depositor.
