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(Plasmid #107671)


Item Catalog # Description Quantity Price (USD)
Plasmid 107671 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Yves Durocher
  • Backbone size w/o insert (bp) 6670
  • Modifications to backbone
    Addition of CD4, biotinylation sequence, 6xhis tags
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    B. microti
  • Insert Size (bp)
  • Mutation
    all potential N-linked glycosylation sites (N-X-S/T, where X is not proline) were systematically removed by substituting alanine for serine/threonine at these sites.
  • Promoter CMV
  • Tag / Fusion Protein
    • ratCD4d3+4,biotinylation sequence, 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EBVori-F (5'-CCACTCAGTGTTGGCAAATG-3')
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Made by gene synthesis. pTT3 was obtained from Yves Durocher, National Research Council of Canada
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BMR1_01g02975-bio-His was a gift from Gavin Wright (Addgene plasmid # 107671 ; ; RRID:Addgene_107671)