pMP4518
(Plasmid
#107778)
-
PurposeExpression of EYFP
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107778 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepBBR1-mcs5
- Backbone size w/o insert (bp) 800
- Total vector size (bp) 6400
-
Vector typeBacterial Expression
-
Selectable markerskanamycin
Growth in Bacteria
-
Bacterial Resistance(s)Gentamicin, 10 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)XL1 Blue
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namepLacZ-EYFP in pBBR1-mcs5
-
SpeciesAequorea victoria (Jellyfish)
-
Insert Size (bp)5600
- Promoter pLacZ
-
Tag
/ Fusion Protein
- pLacZ-EYFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (unknown if destroyed)
- 3′ cloning site Xba I (unknown if destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMP4518 was a gift from Herman Spaink (Addgene plasmid # 107778 ; http://n2t.net/addgene:107778 ; RRID:Addgene_107778) -
For your References section:
Use of green fluorescent protein color variants expressed on stable broad-host-range vectors to visualize rhizobia interacting with plants. Stuurman N, Pacios Bras C, Schlaman HR, Wijfjes AH, Bloemberg G, Spaink HP. Mol Plant Microbe Interact. 2000 Nov;13(11):1163-9. doi: 10.1094/MPMI.2000.13.11.1163. 10.1094/MPMI.2000.13.11.1163 PubMed 11059482
