pPtGE34
(Plasmid #107932)

• Purpose: Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation
• Depositing Lab: David Edgell
• Depositing Organization: University of Western Ontario
  Located in Canada
• Publication: Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021/acssynbio.7b00191.

Backbone

• Vector backbone: pKS diaCas9_sgRNA
• Backbone manufacturer: Per Winge
• Backbone size w/o insert (bp): 8577
• Total vector size (bp): 12392
• Modifications to backbone: Addition of yeast Cen6-ArsH4-His3, origin of transfer, and P. tricornutum ShBle cassette
• Vector type: CRISPR, Synthetic Biology ; Episomal vector for Phaeodactylum tricornutum
• Selectable markers: Zeocin, HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): EPI300
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: OriT
• Alt name: Origin of Transfer
• Insert Size (bp): 771

Gene/Insert 2

• Gene/Insert name: 40SRPS8 Promoter
• Species: Phaeodactylum tricornutum
• Insert Size (bp): 600

Gene/Insert 3

• Gene/Insert name: ShBle
• Alt name: Zeocin resistance gene
• Species: Streptoalloteichus hindustanus
• Insert Size (bp): 375

Gene/Insert 4

• Gene/Insert name: 40SRPS8 Terminator
• Species: Phaeodactylum tricornutum
• Insert Size (bp): 500

Gene/Insert 5

• Gene/Insert name: Cen6-ArsH4-His3
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1349

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 107932-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 107932-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.