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Addgene

pPtGE35
(Plasmid #107999)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 107999 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pKS diaCas9_sgRNA
  • Backbone manufacturer
    Per Winge
  • Backbone size w/o insert (bp) 8577
  • Total vector size (bp) 12925
  • Modifications to backbone
    Addition of yeast Cen6-ArsH4-His3, origin of transfer, P. tricornutum ShBle cassette, and I-TevI nuclease doamin
  • Vector type
    CRISPR, Synthetic Biology ; Episomal vector for Phaeodactylum tricornutum
  • Selectable markers
    Zeocin, HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    OriT
  • Alt name
    Origin of Transfer
  • Insert Size (bp)
    771

Gene/Insert 2

  • Gene/Insert name
    40SRPS8 Promoter
  • Species
    Phaeodactylum tricornutum
  • Insert Size (bp)
    600

Gene/Insert 3

  • Gene/Insert name
    ShBle
  • Alt name
    Zeocin resistance gene
  • Species
    Streptoalloteichus hindustanus
  • Insert Size (bp)
    375

Gene/Insert 4

  • Gene/Insert name
    40SRPS8 Terminator
  • Species
    Phaeodactylum tricornutum
  • Insert Size (bp)
    500

Gene/Insert 5

  • Gene/Insert name
    Cen6-ArsH4-His3
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1349

Gene/Insert 6

  • Gene/Insert name
    I-TevI nuclease and partial linker domain
  • Alt name
    diaTev169(G2S)2
  • Species
    Enterobacteria phage T4T
  • Insert Size (bp)
    528
  • Tag / Fusion Protein
    • Cas9 (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPtGE35 was a gift from David Edgell (Addgene plasmid # 107999 ; http://n2t.net/addgene:107999 ; RRID:Addgene_107999)
  • For your References section:

    An Expanded Plasmid-Based Genetic Toolbox Enables Cas9 Genome Editing and Stable Maintenance of Synthetic Pathways in Phaeodactylum tricornutum. Slattery SS, Diamond A, Wang H, Therrien JA, Lant JT, Jazey T, Lee K, Klassen Z, Desgagne-Penix I, Karas BJ, Edgell DR. ACS Synth Biol. 2018 Jan 8. doi: 10.1021/acssynbio.7b00191. 10.1021/acssynbio.7b00191 PubMed 29298053