Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10797||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4650
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePKC epsilon
SpeciesH. sapiens (human)
Insert Size (bp)2300
Entrez GenePRKCE (a.k.a. PKCE, nPKC-epsilon)
/ Fusion Proteins
- Myr (N terminal on insert)
- FLAG (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII or KpnI? (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Constitutively active. Myr sequence from p60src.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Myr.PKCepsilon.FLAG was a gift from Alex Toker (Addgene plasmid # 10797 ; http://n2t.net/addgene:10797 ; RRID:Addgene_10797)
For your References section:Regulation of novel protein kinase C epsilon by phosphorylation. Cenni V, Doppler H, Sonnenburg ED, Maraldi N, Newton AC, Toker A. Biochem J. 2002 May 1. 363(Pt 3):537-45. 10.1042/0264-6021:3630537 PubMed 11964154