Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #108027)


Item Catalog # Description Quantity Price (USD)
Plasmid 108027 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Yves Durocher
  • Backbone size w/o insert (bp) 6670
  • Modifications to backbone
    Addition of CD4, biotinylation sequence, 6xhis tags
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    B. microti
  • Insert Size (bp)
  • Mutation
    all potential N-linked glycosylation sites (N-X-S/T, where X is not proline) were systematically removed by substituting alanine for serine/threonine at these sites.
  • Promoter CMV
  • Tag / Fusion Protein
    • ratCD4d3+4,biotinylation sequence, 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EBVori-F (5'-CCACTCAGTGTTGGCAAATG-3')
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Made by gene synthesis. pTT3 was obtained from Yves Durocher, National Research Council of Canada
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BMR1_04g05295-bio-His was a gift from Gavin Wright (Addgene plasmid # 108027 ; ; RRID:Addgene_108027)