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Addgene

Vmn2r1-5Hprt-loxP TV
(Plasmid #108079)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108079 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript II SK+
  • Backbone manufacturer
    Agilent Technologies/Stratagene
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 15000
  • Vector type
    Mouse Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1-Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vmn2r1-Hprt (5')-loxP-neo
  • Alt name
    Vmn2r1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    12189
  • Entrez Gene
    Vmn2r1 (a.k.a. Casrl1, EG56544, V2r83)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (destroyed during cloning)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Vmn2r1-5Hprt-loxP TV was a gift from Peter Mombaerts (Addgene plasmid # 108079 ; http://n2t.net/addgene:108079 ; RRID:Addgene_108079)
  • For your References section:

    Subpopulations of vomeronasal sensory neurons with coordinated coexpression of type 2 vomeronasal receptor genes are differentially dependent on Vmn2r1. Akiyoshi S, Ishii T, Bai Z, Mombaerts P. Eur J Neurosci. 2018 Apr;47(7):887-900. doi: 10.1111/ejn.13875. Epub 2018 Mar 25. 10.1111/ejn.13875 PubMed 29465786