pM3GG
(Plasmid #108202)

• Purpose: M. sedula PCNA3
• Depositing Lab: Teruyuki Nagamune
• Publication: Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662. doi: 10.1002/biot.201700662. Epub 2018 Apr 30.

Backbone

• Vector backbone: pET-28b(+)
• Backbone manufacturer: Merck Millipore
• Backbone size w/o insert (bp): 5330
• Total vector size (bp): 6079
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Supplement with 1% glucose is recommended to suppress leaky expression.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: M. sedula PCNA3
• Alt name: MsePCNA3
• Species: Synthetic; Metallosphaera sedula
• Insert Size (bp): 750
• Promoter: T7 promoter
• Tags / Fusion Proteins:
  • His6 tag (N terminal on backbone)
  • His6 tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry