pBsaGSCYP101A1
(Plasmid
#108206)
-
PurposeCYP101A1
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108206 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepHSG398
-
Backbone manufacturerTaKaRa
- Backbone size w/o insert (bp) 2230
- Total vector size (bp) 3503
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCYP101A1
-
Alt nameP450cam
-
SpeciesSynthetic; Pseudomonas putida
-
Insert Size (bp)1300
- Promoter lac promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 Reverse
- 3′ sequencing primer M13 (-40) (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBsaGSCYP101A1 was a gift from Teruyuki Nagamune (Addgene plasmid # 108206 ; http://n2t.net/addgene:108206 ; RRID:Addgene_108206) -
For your References section:
A Stable Artificial Multienzymatic Complex Using a Heterotrimeric Protein From Metallosphaera sedula. Iwata F, Hirakawa H, Nagamune T. Biotechnol J. 2018 Nov;13(11):e1700662. doi: 10.1002/biot.201700662. Epub 2018 Apr 30. 10.1002/biot.201700662 PubMed 29663675
