Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pCR3-vsv-AuroraB L154G/H250Y
(Plasmid #108490)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 108490 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCR3
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AuroraB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1035
  • Mutation
    Analog sensitive L154G/H250Y
  • Entrez Gene
    AURKB (a.k.a. AIK2, AIM-1, AIM1, ARK-2, ARK2, AurB, IPL1, PPP1R48, STK-1, STK12, STK5, aurkb-sv1, aurkb-sv2)
  • Promoter CMV
  • Tag / Fusion Protein
    • VSV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site EcoR1 (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR3-vsv-AuroraB L154G/H250Y was a gift from Susanne Lens (Addgene plasmid # 108490 ; http://n2t.net/addgene:108490 ; RRID:Addgene_108490)
  • For your References section:

    Development of a chemical genetic approach for human aurora B kinase identifies novel substrates of the chromosomal passenger complex. Hengeveld RC, Hertz NT, Vromans MJ, Zhang C, Burlingame AL, Shokat KM, Lens SM. Mol Cell Proteomics. 2012 May;11(5):47-59. doi: 10.1074/mcp.M111.013912. Epub 2012 Jan 20. 10.1074/mcp.M111.013912 PubMed 22267324