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vsv-CenpB 1-166-Sgo1 N61I-GFP
(Plasmid #108499)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108499 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 108499-D50 50 μg of DNA in Tris buffer $495
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DNA 108499-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CenpB-Shugoshin 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2100
  • Mutation
    CenpB 1-166 fusion before Sgo1, no PP2A binding N61I
  • Entrez Gene
    SGO1 (a.k.a. CAID, NY-BR-85, SGO, SGOL1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • VSV (N terminal on insert)
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HinDIII (unknown if destroyed)
  • 3′ cloning site HinDIII (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    vsv-CenpB 1-166-Sgo1 N61I-GFP was a gift from Susanne Lens (Addgene plasmid # 108499 ; http://n2t.net/addgene:108499 ; RRID:Addgene_108499)

  • For your References section:

    Shugoshin-1 balances Aurora B kinase activity via PP2A to promote chromosome bi-orientation. Meppelink A, Kabeche L, Vromans MJ, Compton DA, Lens SM. Cell Rep. 2015 Apr 28;11(4):508-15. doi: 10.1016/j.celrep.2015.03.052. Epub 2015 Apr 16. 10.1016/j.celrep.2015.03.052 PubMed 25892238
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