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Addgene
Shugoshin-1 balances Aurora B kinase activity via PP2A to promote chromosome bi-orientation.
Meppelink A, Kabeche L, Vromans MJ, Compton DA, Lens SM
Cell Rep. 2015 Apr 28;11(4):508-15. doi: 10.1016/j.celrep.2015.03.052. Epub 2015 Apr 16. PubMed Article

Plasmids from Article

ID Plasmid Purpose
108494vsv-Sgo1-GFPexpression of vsv-Sgo1-EGFP
108495vsv-CenpB 1-166-Sgo1-GFPexpression of vsv-CenpB-1-166-Sgo1-EGFP
108496pCR3-vsv-Sgo1 N61Iexpression of vsv-Sgo1 N61I (no PP2A binding)
108497vsv-Sgo1 N61I-GFPexpression of vsv-Sgo1 N61I-EGFP (no PP2A binding)
108498pCR3-vsv-CenpB 1-166-Sgo1 N61Iexpression of vsv-CenpB 1-166-Sgo1 N61I (no PP2A binding)
108499vsv-CenpB 1-166-Sgo1 N61I-GFPexpression of vsv-CenpB 1-166-Sgo1 N61I-EGFP (no PP2A binding)

Antibodies from Article