pCR3-vsv-Incenp 8KQ
(Plasmid #108501)

• Purpose: expression of vsv-INCENP 8KQ
• Depositing Lab: Susanne Lens
• Publication: van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi: 10.1016/j.celrep.2015.06.038. Epub 2015 Jul 9.

Backbone

• Vector backbone: pCR3
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: INCENP
• Species: H. sapiens (human)
• Insert Size (bp): 2757
• Mutation: K548Q, K563Q, K602Q, K609Q, K614Q, K622Q, K630Q, K631Q
• Entrez Gene: INCENP
• Promoter: CMV
• Tag / Fusion Protein:
  • VSV (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • SL-631 vsv-INCENP 8KQ.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Compared to the NCBI reference sequence NM_001040694, this plasmid contains small, in-frame insertions in INCENP after aa 543 and aa 746. These insertions are extra restriction sites for cloning.