pMpGE006
(Plasmid #108722)

• Purpose: Cas9 expression in M.polymorpha
• Depositing Labs: Takayuki Kohchi, Shigeo Sugano
• Publication: Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. doi: 10.1371/journal.pone.0205117. eCollection 2018.

Backbone

• Vector backbone: pMpGWB103
• Backbone manufacturer: Kohchi Lab., Kyoto
• Backbone size w/o insert (bp): 11315
• Total vector size (bp): 15938
• Vector type: Plant Expression, CRISPR
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Atco-Cas9-Pea3ter
• Species: Synthetic
• Insert Size (bp): 4623
• Promoter: MpEFpro

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CACCATGGATAAGAAGTACTCTATCGG
• 3′ sequencing primer: CAGGAAACAGCTATGACCATG

Resource Information

• Supplemental Documents:
  • pMpGE006.gb
• A portion of this plasmid was derived from a plasmid made by: Atco-Cas9-Pea3ter is from Holger Puchta Lab. Fauser et al. (2014) Plant J

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/03/14/277350 for BioRxiv preprint