Skip to main content
Addgene
Open Menu

VEGFR2-YFP
(Plasmid #108853)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108853 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 108853-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 108853-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pBE
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    See Depositor Comments below.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KDR
  • Alt name
    FLK1
  • Alt name
    VEGFR2
  • Species
    H. sapiens (human)
  • GenBank ID
  • Entrez Gene
    KDR (a.k.a. CD309, FLK1, VEGFR, VEGFR2)
  • Tag / Fusion Protein
    • Labeled with eYFP on the C-Terminus via a 3 amino acid (GGS) flexible linker

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Restriction digest results suggest that this plasmid may undergo recombination due to the large insert size. We recommend screening at least 3-5 colonies by restriction digest upon receipt.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VEGFR2-YFP was a gift from Kalina Hristova (Addgene plasmid # 108853 ; http://n2t.net/addgene:108853 ; RRID:Addgene_108853)

  • For your References section:

    VEGFR-2 conformational switch in response to ligand binding. Sarabipour S, Ballmer-Hofer K, Hristova K. Elife. 2016 Apr 7;5:e13876. doi: 10.7554/eLife.13876. 10.7554/eLife.13876 PubMed 27052508
Related items:
Commonly requested with: