pJCSUP35(1-253)
(Plasmid #1089)

• Depositing Lab: Susan Lindquist
• Publication: Glover et al Cell 1997 May 30;89(5):811-9.

Backbone

• Vector backbone: pJC45
• Backbone manufacturer: J. Clos and Brandau, 1994
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SUP35
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 759
• Mutation: amino acids 1 - 253 of SUP35
• GenBank ID: AY553990
• Entrez Gene: SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Ter-Avanesyan et al., 1993
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry