HA-mRIN2
(Plasmid
#108940)
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PurposeMammalian expression of mRIN2
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Depositing Lab
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Depositing OrganizationUniversita di Torino
Located in Italy -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108940 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 108940-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 108940-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonephCMV2
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Backbone manufacturerGenlantis
- Backbone size w/o insert (bp) 4261
- Total vector size (bp) 6838
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRas and Rab interactor 2
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Alt nameRin2
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Alt nameRASSF4
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SpeciesM. musculus (mouse)
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Insert Size (bp)2577
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GenBank ID74030
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Entrez GeneRin2 (a.k.a. 2010003K16Rik, 4632403N06Rik, AW821980, RASSF4)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 108940-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 108940-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA-mRIN2 was a gift from Guido Serini (Addgene plasmid # 108940 ; http://n2t.net/addgene:108940 ; RRID:Addgene_108940) -
For your References section:
The R-Ras/RIN2/Rab5 complex controls endothelial cell adhesion and morphogenesis via active integrin endocytosis and Rac signaling. Sandri C, Caccavari F, Valdembri D, Camillo C, Veltel S, Santambrogio M, Lanzetti L, Bussolino F, Ivaska J, Serini G. Cell Res. 2012 Oct;22(10):1479-501. doi: 10.1038/cr.2012.110. Epub 2012 Jul 24. 10.1038/cr.2012.110 PubMed 22825554
