|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10899||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeYeast Expression, Cre/Lox
Selectable markersLEU2, URA3
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert nameHSC82 URA3
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)4200
Entrez GeneHSC82 (a.k.a. YMR186W, HSP90)
- Cloning method Restriction Enzyme
- 5′ cloning site SacII (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Yeast integrating plasmid. URA3 flanked by loxP sites. HSC82 outside of the loxP sites.
Insert from plasmid 7852 pUG72-HSC82out.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:7861 pRS305-pUG72-HSC82out was a gift from Susan Lindquist (Addgene plasmid # 10899 ; http://n2t.net/addgene:10899 ; RRID:Addgene_10899)
For your References section:Hsp90 potentiates the rapid evolution of new traits: drug resistance in diverse fungi. Cowen LE, Lindquist S. Science. 2005 Sep 30. 309(5744):2185-9. 10.1126/science.1118370 PubMed 16195452