DEP-GFP
(Plasmid #109043)

• Purpose: Expression of the Dishevelled DEP domain
• Depositing Lab: Mariann Bienz
• Publication: Gammons et al Mol Cell. 2016 Oct 6;64(1):92-104. doi: 10.1016/j.molcel.2016.08.026. Epub 2016 Sep 29.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dishevelled segment polarity protein 2
• Species: H. sapiens (human)
• Mutation: Dvl2 DEP domain
• Entrez Gene: DVL2
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dvl2-GFP was provided by Trevor Dale (Cardiff), who published the construction of this plasmid in 1999 (Smalley et al, EMBO J; PMID: 10329628)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry