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DEP-GFP
(Plasmid #109043)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 109043 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dishevelled segment polarity protein 2
  • Species
    H. sapiens (human)
  • Mutation
    Dvl2 DEP domain
  • Entrez Gene
    DVL2
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dvl2-GFP was provided by Trevor Dale (Cardiff), who published the construction of this plasmid in 1999 (Smalley et al, EMBO J; PMID: 10329628)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DEP-GFP was a gift from Mariann Bienz (Addgene plasmid # 109043 ; http://n2t.net/addgene:109043 ; RRID:Addgene_109043)
  • For your References section:

    Wnt Signalosome Assembly by DEP Domain Swapping of Dishevelled. Gammons MV, Renko M, Johnson CM, Rutherford TJ, Bienz M. Mol Cell. 2016 Oct 6;64(1):92-104. doi: 10.1016/j.molcel.2016.08.026. Epub 2016 Sep 29. 10.1016/j.molcel.2016.08.026 PubMed 27692984