pYC6H-TRP1URA3
(Plasmid #11009)

• Depositing Lab: Francisco Malagon
• Publication: Unpublished, Paco (unpublished)

Backbone

• Vector backbone: pYC6-CT/HIS3 hybrid
• Backbone size w/o insert (bp): 3786
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pgal1TRP1URA3
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 2240
• Mutation: ORF-TRP1 fused to ORF-URA without stop codon
• Tags / Fusion Proteins:
  • his6 (C terminal on backbone)
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NaeI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified mutations A160S and K173R in the URA3 sequence. These mutations were not intentional and the depositing scientist states that the plasmid was tested for complementation of URA and TRP phenotypes and for production of a single transcript.