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PurposeExpression of human RAPGEF2 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110162 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRAPGEF2 (Rap guanine nucleotide exchange factor 2
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Alt namePDZGEF1
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SpeciesH. sapiens (human)
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Entrez GeneRAPGEF2 (a.k.a. CNrasGEF, NRAPGEP, PDZ-GEF1, PDZGEF1, RA-GEF, RA-GEF-1, RAGEF, Rap-GEP, nRap GEP)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-HA-RAPGEF2 was a gift from Daniele Guardavaccaro (Addgene plasmid # 110162 ; http://n2t.net/addgene:110162 ; RRID:Addgene_110162) -
For your References section:
Control of epithelial cell migration and invasion by the IKKbeta- and CK1alpha-mediated degradation of RAPGEF2. Magliozzi R, Low TY, Weijts BG, Cheng T, Spanjaard E, Mohammed S, van Veen A, Ovaa H, de Rooij J, Zwartkruis FJ, Bos JL, de Bruin A, Heck AJ, Guardavaccaro D. Dev Cell. 2013 Dec 9;27(5):574-85. doi: 10.1016/j.devcel.2013.10.023. Epub 2013 Nov 27. 10.1016/j.devcel.2013.10.023 PubMed 24290981
