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pNIC-ZB-SPRTN-E112A-FL
(Plasmid #110218)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 110218 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pNIC-ZB
  • Backbone manufacturer
    Opher Gileadi, Addgene plasmid #26107
  • Modifications to backbone
    vector treated with BsaI, then with T4 DNA polymerase in presence of dGTP
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Growth Strain Other: Rosetta DE3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SPRTN
  • Alt name
    C1orf124
  • Alt name
    DVC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1494
  • Mutation
    E112A, P296L (see depositor comments below)
  • Entrez Gene
    SPRTN (a.k.a. C1orf124, DVC1, PRO4323, spartan)
  • Tags / Fusion Proteins
    • His6 (N terminal on insert)
    • ZB (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

According to the large datasets deposited in various databses like: dbSNP, 1000 Genomes, the ExAC and the gnomAD browser, the variant c.887C>T, p.Pro296Leu is found roughly in 50% of general world population. Moreover, according to the large-scale sequencing studies involving more than 135 000 healthy individuals deposited in The Genome Aggregation Database (gnomAD), it is more prevalent than the previously assigned reference allele in the European population, with a minor allele frequency of 0.69: http://gnomad.broadinstitute.org/variant/1-231488524-C-T
Taken this into account, this variant (P296L) is s actually the accurate reference (wild-type) allele, and thus this variant can not be the cause of any Mendelian disease. This is the reason why all our vectors bear this variant.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNIC-ZB-SPRTN-E112A-FL was a gift from Kristijan Ramadan (Addgene plasmid # 110218 ; http://n2t.net/addgene:110218 ; RRID:Addgene_110218)
  • For your References section:

    Metalloprotease SPRTN/DVC1 Orchestrates Replication-Coupled DNA-Protein Crosslink Repair. Vaz B, Popovic M, Newman JA, Fielden J, Aitkenhead H, Halder S, Singh AN, Vendrell I, Fischer R, Torrecilla I, Drobnitzky N, Freire R, Amor DJ, Lockhart PJ, Kessler BM, McKenna GW, Gileadi O, Ramadan K. Mol Cell. 2016 Nov 17;64(4):704-719. doi: 10.1016/j.molcel.2016.09.032. Epub 2016 Oct 27. 10.1016/j.molcel.2016.09.032 PubMed 27871366