|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||110251||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerOpher Gileadi (Addgene plasmid # 26103)
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Alt namekelch like family member 3
SpeciesH. sapiens (human)
Entrez GeneKLHL3 (a.k.a. PHA2D)
- Promoter T7 - lacO
/ Fusion Protein
- His-TEV (N terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Use BL21(DE3)-R3-pRARE2 for expression. 4CH9: https://www.thesgc.org/structures/4CH9.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:KLHL3 (KLHL3A-c003) was a gift from Nicola Burgess-Brown (Addgene plasmid # 110251 ; http://n2t.net/addgene:110251 ; RRID:Addgene_110251)