pSKC11 - UTRQ
PurposeMonitor the expression of Luciferase driven by the NRAS 5'-UTR.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||110490||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 2680
Growth in Bacteria
Gene/Insert nameNRAS 5'-UTR (bp 1-254)
SpeciesH. sapiens (human)
Insert Size (bp)254
Entrez GeneNRAS (a.k.a. ALPS4, CMNS, N-ras, NCMS, NRAS1, NS6)
- Promoter T7
/ Fusion Protein
- Firefly luciferase (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer M13-fwd
- 3′ sequencing primer M13-rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSKC11 - UTRQ was a gift from Shankar Balasubramanian (Addgene plasmid # 110490 ; http://n2t.net/addgene:110490 ; RRID:Addgene_110490)
For your References section:An RNA G-quadruplex in the 5' UTR of the NRAS proto-oncogene modulates translation. Kumari S, Bugaut A, Huppert JL, Balasubramanian S. Nat Chem Biol. 2007 Apr;3(4):218-21. doi: 10.1038/nchembio864. Epub 2007 Feb 25. 10.1038/nchembio864 PubMed 17322877