T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB
PurposeCRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||110547||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2412
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
SpeciesSynthetic; Streptococcus pyrogenes
- Promoter pBAD
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB was a gift from Ichiro Matsumura (Addgene plasmid # 110547 ; http://n2t.net/addgene:110547 ; RRID:Addgene_110547)