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p182 pK7-HDAC1 mut (GFP)
(Plasmid #11055)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11055 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HDAC1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1300
  • Mutation
    Deletion of aa 1-56: deacetylase domain deletion
  • Entrez Gene
    Hdac1 (a.k.a. HD1, Hdac1-ps, MommeD5, RPD3)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Addgene sequencing data finds T189A and D332G substitutions. These are not known to affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p182 pK7-HDAC1 mut (GFP) was a gift from Ramesh Shivdasani (Addgene plasmid # 11055 ; http://n2t.net/addgene:11055 ; RRID:Addgene_11055)
  • For your References section:

    Regulation of mammalian epithelial differentiation and intestine development by class I histone deacetylases. Tou L, Liu Q, Shivdasani RA. Mol Cell Biol. 2004 Apr . 24(8):3132-9. 10.1128/MCB.24.8.3132-3139.2004 PubMed 15060137