Purpose(Empty Backbone) Plasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transposition
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||110738||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5279
Vector typeInsect Expression
- Promoter Polyhedrin and p10 promoters
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Gentamicin
Copy numberHigh Copy
- Cloning method Restriction Enzyme
Terms and Licenses
- Not Available to Industry
pFBDM was derived from pFastBacDUAL (Invitrogen), which enables the production of recombinant proteins in insect cells by baculovirus. This pFBDM vector is used mostly in our lab for the production of various target proteins composed of one or two polypeptides.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFBDM was a gift from Tim Richmond (Addgene plasmid # 110738 ; http://n2t.net/addgene:110738 ; RRID:Addgene_110738)
For your References section:Protein complex expression by using multigene baculoviral vectors. Fitzgerald DJ, Berger P, Schaffitzel C, Yamada K, Richmond TJ, Berger I. Nat Methods. 2006 Dec;3(12):1021-32. doi: 10.1038/nmeth983. 10.1038/nmeth983 PubMed 17117155