Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pLIX403-hNOS2
(Plasmid #110800)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 110800 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLIX_403
  • Backbone manufacturer
    David Root (Addgene plasmid # 41395)
  • Backbone size w/o insert (bp) 9396
  • Total vector size (bp) 12857
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NOS2
  • Alt name
    nitric oxide synthase 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3461
  • Entrez Gene
    NOS2 (a.k.a. HEP-NOS, INOS, NOS, NOS2A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer LNCX
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIX403-hNOS2 was a gift from Edward Morgan (Addgene plasmid # 110800 ; http://n2t.net/addgene:110800 ; RRID:Addgene_110800)
  • For your References section:

    Nitric oxide-regulated proteolysis of human CYP2B6 via the ubiquitin-proteasome system. Lee CM, Tripathi S, Morgan ET. Free Radic Biol Med. 2017 Jul;108:478-486. doi: 10.1016/j.freeradbiomed.2017.04.015. Epub 2017 Apr 17. 10.1016/j.freeradbiomed.2017.04.015 PubMed 28427998