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pRP(Exp)-CMV> :dTomato:IRES:Puro
(Plasmid #110832)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 110832 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    vector builder
  • Backbone size w/o insert (bp) 4726
  • Total vector size (bp) 5671
  • Vector type
    non-viral gene expression vector
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dTomato Tag
  • Alt name
    dT
  • Insert Size (bp)
    945
  • Promoter CMV
  • Tag / Fusion Protein
    • IRES (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer dTomato-reverse primer: CCTCGCCCTTGCTCACCAT
  • 3′ sequencing primer CMV-forward primer: TTGACGCAAATGGGCGGTAGG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    vector builder

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRP(Exp)-CMV> :dTomato:IRES:Puro was a gift from Ande Satyanarayana (Addgene plasmid # 110832 ; http://n2t.net/addgene:110832 ; RRID:Addgene_110832)
  • For your References section:

    The tumor secretory factor ZAG promotes white adipose tissue browning and energy wasting. Elattar S, Dimri M, Satyanarayana A. FASEB J. 2018 Mar 23:fj201701465RR. doi: 10.1096/fj.201701465RR. 10.1096/fj.201701465RR PubMed 29570397