pLenti6.3-OGDH_wobble
(Plasmid #110938)

• Purpose: OGDH ORF containing silent wobble mutations insensitive to corresponding shRNAs
• Depositing Lab: William Hahn
• Publication: Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):E3434-E3443. doi: 10.1073/pnas.1617922114. Epub 2017 Apr 10.

Backbone

• Vector backbone: pLenti6.3/V5-DEST
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 9387
• Vector type: Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: OGDH wobble cDNA
• Alt name: KGDH
• Species: H. sapiens (human)
• Insert Size (bp): 3071
• Mutation: 15 silent wobble mutations corresponding to three shRNA sites
• GenBank ID: NM_002541.3
• Entrez Gene: OGDH (a.k.a. AKGDH, E1k, E1o, KGD1, OGDC, OGDH-E1, OGDH2, OGDHD)
• Promoter: SV40

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry