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PurposeHelper plasmid containing Cre-recombinase gene, which is expressed in DH10MultiBac cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111187 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBAD22
- Total vector size (bp) 6427
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Modifications to backboneThe ampicillin resistance marker of pBAD22 was inactivated by digestion. The zeocin resistance marker was ligated yielding pBADZ.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCre recombinase
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Tag
/ Fusion Protein
- His tag (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The protocol for preparation of electro-competent cells of DH10MultiBacCre,which carry the plasmid of pBADZ HisCre and enable the production of Cre recombinase, is described in Fitzgerald et al. (2006).
http://www.nature.com/articles/nmeth983
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBADZ-HisCre was a gift from Tim Richmond (Addgene plasmid # 111187 ; http://n2t.net/addgene:111187 ; RRID:Addgene_111187) -
For your References section:
Baculovirus expression system for heterologous multiprotein complexes. Berger I, Fitzgerald DJ, Richmond TJ. Nat Biotechnol. 2004 Dec;22(12):1583-7. doi: 10.1038/nbt1036. Epub 2004 Nov 28. 10.1038/nbt1036 PubMed 15568020
Map uploaded by the depositor.
