pre-Ubc4 BrG pUC19
(Plasmid #111322)

• Purpose: for transcription to Ubc4 pre-mRNA
• Depositing Lab: Christine Guthrie
• Publication: Abelson et al Methods Enzymol. 2010;472:31-40. doi: 10.1016/S0076-6879(10)72017-6.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2696
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UBC4
• Species: S. cerevisiae (budding yeast)
• Mutation: intron has branch-site A to G mutation
• Entrez Gene: UBC4 (a.k.a. YBR082C)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry