Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMT
(Plasmid
#111400)
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PurposeProtein expression in E.Coli
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111400 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens]
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SpeciesH. sapiens (human)
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MutationKv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L540A
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Entrez GeneKCNQ4 (a.k.a. DFNA2, DFNA2A, KV7.4)
- Promoter T7
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Tags
/ Fusion Proteins
- His Tag (N terminal on insert)
- MBP tag (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 Forward
- 3′ sequencing primer T7 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid contains L540A mutation
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMT was a gift from Dan Minor (Addgene plasmid # 111400 ; http://n2t.net/addgene:111400 ; RRID:Addgene_111400) -
For your References section:
A Calmodulin C-Lobe Ca(2+)-Dependent Switch Governs Kv7 Channel Function. Chang A, Abderemane-Ali F, Hura GL, Rossen ND, Gate RE, Minor DL Jr.. Neuron. 2018 Feb 21;97(4):836-852.e6. doi: 10.1016/j.neuron.2018.01.035. Epub 2018 Feb 8. 10.1016/j.neuron.2018.01.035 PubMed 29429937
Map uploaded by the depositor.
